Invasive behaviour of mouse primordial germ cells in vitro.

The effect of BMP4 on mouse embryonic stem cell proliferation and differentiation into primordial germ cells

Background and Aim: Artificial gamete production from stem cells is a novel strategy for treatment of infertility. Among various stem cell sources, embryonic stem cells (ESC) can be considered as an appropriate source for in vitro formation of germ cells. In this study we evaluated the effect of BMP4 on proliferation and differentiation of mouse embryonic stem cells into primordial germ cells (...

Functional Concentrations of BMP4 on Differentiation of Mouse Embryonic Stem Cells to Primordial Germ Cells

Review of Differentiation and Proliferation of Primordial Germ Cells in Culture

Primordial germ cells (PGCs) are highly specialized cell population that arises from the epiblast in vivo. There are three critical steps in the life cycle of these cells: 1-Specification 2-migration and proliferation 3-prenatal and postnatal sex specific development. Specification of germ cells in epiblast occurs due to signals secreted from extraembryonic tissues. Primordial germ cells are re...

In Vitro Production of Germ Cells from Stem Cells: Hypes and Hopes

Several lines of evidence have reported that mouse ESCs can successfully differentiate into primordial germ cells (PGC) as well as into mature male and female gametes. Human ESCs and adult stem cells (ASCs) can also differentiate into PGCs. Differentiation of ESCs into germ cells of various stages seems to be a spontaneous and quick process, probably due to the nature of ESCs themselves and the...

Comparison of Germ Cell Gene Expressions in Spontaneous Monolayer versus Embryoid Body Differentiation of Mouse Embryonic Stem Cells toward Germ Cells

Objective Genetic and morphologic similarities between mouse embryonic stem cell (ESCs) and Primordial Germ Cell (PGCs) make it difficult to distinguish the two cell types in in vitro differentiation. Using the expression of specific markers of germ cells that are not expressed or expressed at low levels in ESCs, can help recognizing in vitro differentiated cells MaterialsAndMethods In this stu...